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Performance comparison of Agilent new SureSelect All Exon v8 probes with v7 probes for exome sequencing.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2000-
    • Subject Terms:
    • Abstract:
      Exome sequencing is becoming a routine in health care, because it increases the chance of pinpointing the genetic cause of an individual patient's condition and thus making an accurate diagnosis. It is important for facilities providing genetic services to keep track of changes in the technology of exome capture in order to maximize throughput while reducing cost per sample. In this study, we focused on comparing the newly released exome probe set Agilent SureSelect Human All Exon v8 and the previous probe set v7. In preparation for higher throughput of exome sequencing using the DNBSEQ-G400, we evaluated target design, coverage statistics, and variants across these two different exome capture products. Although the target size of the v8 design has not changed much compared to the v7 design (35.24 Mb vs 35.8 Mb), the v8 probe design allows you to call more of SNVs (+ 3.06%) and indels (+ 8.49%) with the same number of raw reads per sample on the common target regions (34.84 Mb). Our results suggest that the new Agilent v8 probe set for exome sequencing yields better data quality than the current Agilent v7 set.
      (© 2022. The Author(s).)
    • References:
      Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168)
      Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19096-101. (PMID: 19861545)
      BMC Cancer. 2016 Aug 30;16:692. (PMID: 27578032)
      Sci Rep. 2020 Feb 24;10(1):3235. (PMID: 32094380)
      NPJ Genom Med. 2021 Aug 12;6(1):66. (PMID: 34385467)
      Sci Rep. 2017 Apr 13;7(1):885. (PMID: 28408746)
      J Clin Med. 2020 Nov 13;9(11):. (PMID: 33202991)
      Genet Med. 2018 Oct;20(10):1122-1130. (PMID: 29446766)
      Sci Rep. 2020 Feb 6;10(1):2057. (PMID: 32029882)
      Methods Mol Biol. 2009;563:123-40. (PMID: 19597783)
      Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
      Sci Rep. 2016 Feb 11;6:20948. (PMID: 26864517)
      Sci Rep. 2015 Aug 03;5:12742. (PMID: 26235669)
      BMC Bioinformatics. 2019 Jun 17;20(1):342. (PMID: 31208315)
      Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
      Sci Rep. 2016 May 25;6:26732. (PMID: 27220682)
      Mol Genet Genomics. 2021 May;296(3):653-663. (PMID: 33694043)
      Sci Rep. 2022 Jan 12;12(1):609. (PMID: 35022470)
    • Grant Information:
      075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation; 075-15-2019-1789 Ministry of Science and Higher Education of the Russian Federation
    • Contributed Indexing:
      Keywords: Agilent SureSelect; BGI; Enrichment quality; Exome sequencing; MGISEQ; NGS; Variant calling; WES
    • Publication Date:
      Date Created: 20220812 Date Completed: 20220816 Latest Revision: 20220816
    • Publication Date:
      20220816
    • Accession Number:
      PMC9375261
    • Accession Number:
      10.1186/s12864-022-08825-w
    • Accession Number:
      35962321