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Vascular Pool of Releasable Soluble VEGF Receptor-1 (sFLT1) in Women With Previous Preeclampsia and Uncomplicated Pregnancy

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  • Additional Information
    • Publication Information:
      Bethesda, MD: Endocrine Society, 2014.
    • Publication Date:
      2014
    • Original Material:
      INIST-CNRS
    • Abstract:
      Context: Research examining the source of excess soluble fms-like tyrosine kinase 1 (sFLT1) in preeclampsia has focused on the placenta. The potential contribution of the releasable store of sFLT1 in the systemic vasculature is unknown. Objective: We asked whether the nonplacental releasable store of sFLT1 is larger in women with previous preeclampsia than in women with a previous uncomplicated pregnancy. Design: We administered heparin to nulligravid women and to women with previous preeclampsia or a previous uncomplicated pregnancy. We compared post-heparin sFLT1 concentrations with those observed in uncomplicated pregnancy and preeclampsia. Setting: The study was performed at Magee-Womens Hospital. Patients: Participants included nulligravidas (n = 8), women 6-24 months postpartum (previous uncomplicated pregnancy, n = 16; previous preeclampsia, n = 15), and pregnant women (uncomplicated pregnancy, n = 30; preeclampsia, n = 25). Intervention: Nonpregnant women received an unfractionated heparin bolus. Main Outcome Measures: Pre- and post-heparin plasma sFLT1, placental growth factor, and vascular endothelial growth factor were measured. Results: In nonpregnant women, heparin increased plasma sFLT1 by 250-fold (P < .01), increased placental growth factor by 7-fold (P < .01), and decreased free vascular endothelial growth factor (P < .01). These changes did not differ between nulligravidas, women with previous preeclampsia, and women with a previous uncomplicated pregnancy. Post-heparin sFLT1 in nonpregnant women was higher than sFLT1 in uncomplicated pregnancy, but lower than sFLT1 in preeclampsia. Baseline and post-heparin sFLT1 were positively correlated (r2 = 0.19; P< .01). Heparin increased the concentration of the 100-kDa sFLT1 isoform. Adding heparin to whole blood or plasma did not increase sFLT1. Conclusions: Nonpregnant women have a significant vascular store of releasable sFLT1. The size of this store does not differ between women with previous preeclampsia vs women with previous uncomplicated pregnancy.
    • File Description:
      text
    • Author Affiliations:
      Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota 55905, United States
      Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, United States
      Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, United States
      Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, United States
      Department of Epidemiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States
      Graduate School of Public Health , University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States
      Clinical and Translational Science Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
      Department of Environmental and Occupational Health, United States
    • ISSN:
      0021-972X
    • Rights:
      Copyright 2015 INIST-CNRS
      CC BY 4.0
      Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
    • Notes:
      Endocrinopathies

      Vertebrates : anatomy and physiology, studies on body, several organs or systems

      Vertebrates : endocrinology
    • Accession Number:
      edscal.28376233