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Academic Journal

Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier.

  • Authors : Chung J; Samsung Genome Institute, Samsung Medical Center, Seoul, 06351, South Korea.; Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, South Korea.

Subjects: Gene Library*; Biomarkers/Biomarkers/Biomarkers/*analysis ; DNA/DNA/DNA/*analysis

  • Source: BMC genomics [BMC Genomics] 2019 Mar 14; Vol. 20 (1), pp. 216. Date of Electronic Publication: 2019 Mar 14.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries.

  • Authors : Omelina ES; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia.; Ivankin AV

Subjects: Gene Library* ; Plasmids*; DNA/DNA/DNA/*genetics

  • Source: BMC genomics [BMC Genomics] 2019 Jul 11; Vol. 20 (Suppl 7), pp. 536. Date of Electronic Publication: 2019 Jul 11.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing.

  • Authors : Aigrain L; Wellcome Trust Sanger Institute, Wellcome Trust Campus, Hinxton, Cambs, CB10 1SA, UK. .; Gu Y

Subjects: Gene Library*; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

  • Source: BMC genomics [BMC Genomics] 2016 Jun 13; Vol. 17, pp. 458. Date of Electronic Publication: 2016 Jun 13.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

  • Authors : Pellegrino M; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.; Sciambi A

Subjects: Gene Expression Profiling*/Gene Expression Profiling*/Gene Expression Profiling*/methods ; High-Throughput Nucleotide Sequencing*/High-Throughput Nucleotide Sequencing*/High-Throughput Nucleotide Sequencing*/methods ; Polymerase Chain Reaction*

  • Source: BMC genomics [BMC Genomics] 2016 May 17; Vol. 17, pp. 361. Date of Electronic Publication: 2016 May 17.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

A Sequel to Sanger: amplicon sequencing that scales.

  • Authors : Hebert PDN; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON, N1G 2W1, Canada. .; Braukmann TWA

Subjects: Arthropods/Arthropods/Arthropods/*genetics ; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

  • Source: BMC genomics [BMC Genomics] 2018 Mar 27; Vol. 19 (1), pp. 219. Date of Electronic Publication: 2018 Mar 27.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries.

  • Authors : Shagin DA; Pirogov Russian National Research Medical University, Moscow, Russia.; Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.

Subjects: Gene Library*; DNA Primers/DNA Primers/DNA Primers/*genetics ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

  • Source: BMC genomics [BMC Genomics] 2017 Jun 05; Vol. 18 (1), pp. 440. Date of Electronic Publication: 2017 Jun 05.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Target enrichment using parallel nanoliter quantitative PCR amplification.

Subjects: Polymerase Chain Reaction*; Cell Line, Tumor ; DNA/DNA/DNA/analysis

  • Source: BMC genomics [BMC Genomics] 2014 Mar 10; Vol. 15, pp. 184. Date of Electronic Publication: 2014 Mar 10.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Determination of haplotypes at structurally complex regions using emulsion haplotype fusion PCR.

  • Authors : Tyson J; School of Biology, University of Nottingham, Queen's Medical Centre, Nottingham, NG7 2UH, UK. ; Armour JA

Subjects: Genome, Human/Genome, Human/Genome, Human/*genetics ; Haplotypes/Haplotypes/Haplotypes/*genetics ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

  • Source: BMC genomics [BMC Genomics] 2012 Dec 11; Vol. 13, pp. 693. Date of Electronic Publication: 2012 Dec 11.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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