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Academic Journal

ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples.

  • Authors : Abellan-Schneyder I; Core Facility Microbiome, ZIEL - Institute for Food & Health, Technische Universität München, Freising, Germany.; Schusser AJ

Subjects: Biomass*; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods ; RNA, Ribosomal, 16S/RNA, Ribosomal, 16S/RNA, Ribosomal, 16S/*genetics

  • Source: BMC microbiology [BMC Microbiol] 2021 Dec 18; Vol. 21 (1), pp. 349. Date of Electronic Publication: 2021 Dec 18.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium:

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Academic Journal

Next-generation sequencing using microfluidic PCR enrichment for molecular autopsy.

  • Authors : Raju H; Cardiovascular Sciences Research Centre, Molecular and Clinical Sciences Research Institute, St George's University of London, London, SW17 0RE, UK. .; Health Cardiology, Faculty of Medicine & Health Sciences, Macquarie University, Suite 203, 2 Technology Place, Sydney, NSW, 2109, Australia. .

Subjects: DNA Mutational Analysis* ; High-Throughput Nucleotide Sequencing* ; Microfluidic Analytical Techniques*

  • Source: BMC cardiovascular disorders [BMC Cardiovasc Disord] 2019 Jul 23; Vol. 19 (1), pp. 174. Date of Electronic Publication: 2019 Jul 23.Publisher: BioMed Central Country of Publication: England NLM ID: 100968539 Publication Model: Electronic Cited Medium:

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Academic Journal

Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier.

  • Authors : Chung J; Samsung Genome Institute, Samsung Medical Center, Seoul, 06351, South Korea.; Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, South Korea.

Subjects: Gene Library*; Biomarkers/Biomarkers/Biomarkers/*analysis ; DNA/DNA/DNA/*analysis

  • Source: BMC genomics [BMC Genomics] 2019 Mar 14; Vol. 20 (1), pp. 216. Date of Electronic Publication: 2019 Mar 14.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

DUGMO: tool for the detection of unknown genetically modified organisms with high-throughput sequencing data for pure bacterial samples.

  • Authors : Hurel J; ANSES, Laboratoire de Ploufragan, GVB unit, 22440, Ploufragan, France.; Schbath S

Subjects: Bacteria/Bacteria/Bacteria/*chemistry ; Computational Biology/Computational Biology/Computational Biology/*methods ; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods

  • Source: BMC bioinformatics [BMC Bioinformatics] 2020 Jul 06; Vol. 21 (1), pp. 284. Date of Electronic Publication: 2020 Jul 06.Publisher: BioMed Central Country of Publication: England NLM ID: 100965194 Publication Model: Electronic Cited Medium:

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Academic Journal

Comparing mutation calls in fixed tumour samples between the affymetrix OncoScan® array and PCR based next-generation sequencing.

  • Authors : Wood HM; Pathology and Tumour Biology, Leeds Institute of Cancer and Pathology, Leeds, UK. .; Foster JM

Subjects: High-Throughput Nucleotide Sequencing* ; Oligonucleotide Array Sequence Analysis* ; Polymerase Chain Reaction*

  • Source: BMC medical genomics [BMC Med Genomics] 2017 Mar 18; Vol. 10 (1), pp. 17. Date of Electronic Publication: 2017 Mar 18.Publisher: BioMed Central Country of Publication: England NLM ID: 101319628 Publication Model: Electronic Cited Medium:

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Academic Journal

Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries.

  • Authors : Omelina ES; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia.; Ivankin AV

Subjects: Gene Library* ; Plasmids*; DNA/DNA/DNA/*genetics

  • Source: BMC genomics [BMC Genomics] 2019 Jul 11; Vol. 20 (Suppl 7), pp. 536. Date of Electronic Publication: 2019 Jul 11.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

Bovine milk microbiome: a more complex issue than expected.

  • Authors : Taponen S; Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. .; McGuinness D

Subjects: Microbiota*; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods ; Milk/Milk/Milk/*microbiology

  • Source: Veterinary research [Vet Res] 2019 Jun 06; Vol. 50 (1), pp. 44. Date of Electronic Publication: 2019 Jun 06.Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium:

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Academic Journal

Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing.

  • Authors : Aigrain L; Wellcome Trust Sanger Institute, Wellcome Trust Campus, Hinxton, Cambs, CB10 1SA, UK. .; Gu Y

Subjects: Gene Library*; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

  • Source: BMC genomics [BMC Genomics] 2016 Jun 13; Vol. 17, pp. 458. Date of Electronic Publication: 2016 Jun 13.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.

  • Authors : Pellegrino M; Mission Bio, Inc., 953 Indiana St., San Francisco, California, 94107, USA.; Sciambi A

Subjects: Gene Expression Profiling*/Gene Expression Profiling*/Gene Expression Profiling*/methods ; High-Throughput Nucleotide Sequencing*/High-Throughput Nucleotide Sequencing*/High-Throughput Nucleotide Sequencing*/methods ; Polymerase Chain Reaction*

  • Source: BMC genomics [BMC Genomics] 2016 May 17; Vol. 17, pp. 361. Date of Electronic Publication: 2016 May 17.Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium:

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Academic Journal

A high-throughput sequencing assay to comprehensively detect and characterize unicellular eukaryotes and helminths from biological and environmental samples.

  • Authors : Cannon MV; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.; Bogale H

Subjects: Food Parasitology/Food Parasitology/Food Parasitology/*methods ; Helminths/Helminths/Helminths/*classification ; High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/High-Throughput Nucleotide Sequencing/*methods

  • Source: Microbiome [Microbiome] 2018 Oct 29; Vol. 6 (1), pp. 195. Date of Electronic Publication: 2018 Oct 29.Publisher: BioMed Central Country of Publication: England NLM ID: 101615147 Publication Model: Electronic Cited Medium:

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